c-Fos: A Converging Point for Intracellular Signalling and Transcription Regulation

The c-Fos proto-oncogene is a converging point for multiple signal transduction pathways from the extracellular milieu into the nucleus. As a member of the Fos family of transcription factors, c-Fos is characterized by a basic leucine zipper for dimerisation and DNA-binding and contains a transactivation domain at the C-terminus.  c-Fos heterodimerizes with c-jun and other related proteins to form Activator Protein-1 (AP-1) complexes that mediate transcription via AP-1 sites. The AP-1 consensus binding site is a palindromic DNA motif TGA(G/C)TCA found in promoters and enhancers.

c-Fos expression can be triggered by a wide variety of cell-extrinsic and cell-intrinsic signals, including serum, growth factors, tumor promoters, cytokines, and UV radiation. It is considered to be an immediate early gene (IEG) as c-Fos mRNA and protein is rapidly and transiently transcribed upon stimulation, without the need for de novo protein synthesis. c-Fos contains multiple transactivation modules in the N- and C-terminal regions. Its activity is also regulated by kinases, such as MAPK, cdc2, PKA or PKC and its phosphorylation has an influence on its protein stability and transcriptional activity.

c-Fos is ubiquitously expressed in many cells. As a neuronal IEG, c-Fos has been widely used to facilitate neuronal activity mapping. c-Fos is also involved in various cellular events, including cell proliferation, differentiation, growth, angiogenesis and apoptosis. Dysregulation of the proto-oncogene therefore plays an important factor in carcinogenesis. It can lead to the loss of cell polarity and epithelial-mesenchymal transition to promote tumor invasion and metastasis.

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Image: Immunohistochemistry analysis of human brain using free floating sections. Immunoreactivity detected in the nucleoplasm in neuronal cells in the cerebral cortex using our anti-c-Fos antibody.

Antibodies available to purchase via DISCOVERY Antibodies

Anti-c-Fos antibody  (catalogue number: crb4005003f)

c-Fos peptide (catalogue number: crb1200272e)

Alexa Fluor® 488 Anti-c-Fos antibody (catalogue number: crb2115003e)

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