qM FRET kits
Single label homogeneous TRF modulation assays based on qFM TRF1
CRB now offers two homogeneous assays for measuring GTP pathways for GEF mediated GTP exchange and GTP hydrolysis based on residual concentration monitoring using a GTP specific antibody. The assays enable screening of possible candidate inhibitors for both GTP association and hydrolysis with small and large GTPases. The GTPases act as a "molecular switch", shifting between GDP bound (inactive) and GTP bound (active) conformations. The cycle is controlled by guanine nucleotide exchange factor (GEF), and GTPase activating protein (GAP). Researchers can now screen the full GTP cycle effecting small molecule inhibitors by specifically monitoring GTP levels.
- GTP association and dissociation measurement for GEF
- Kinetic and endpoint readouts of association and dissociation
- A GTP Hydrolysis measurement for GAP pathways
- Determination of residual GTP from GAP pathways