Anti-Phospho-GluR1 (pS845) antibody

Description

Recognises the GluR1 subunit of AMPAR with a phosphorylated serine at position 845

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Catalogue number crb2005048
Antibody Anti-Phospho-GluR1 (pS845) antibody
Antigen Peptide Anti-phospho-GluR1 (pS845) antibody
Protein ID UniProtKB - P35438
Aliases GluA1
Cross-Reactivity Human, rat, mouse, goat
Host Species Anti-Rabbit
Antibody Type Polyclonal
Label Unconjugated
Concentration 1mg/ml
Validation 1:1000 (WB), 1:1000 (ELISA)
Target phosphorylated GluR1 (pS845)
Disease Area Neurodegenerative
Family AMPA receptor
Specificity Protein
Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4oC for short term storage and -20oC for long term storage. Avoid repeated freeze/ thaw cycles.
References

Khatib, T., Whiting, A., Chisholm, D., Redfern, C., Müller, B. and McCaffery, P. (2019). A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor. Mol Neurobiol. PMID: 30972628

 

Spaethling, J., Le, L. and Meaney, D. (2012). NMDA receptor mediated phosphorylation of GluR1 subunits contributes to the appearance of calcium-permeable AMPA receptors after mechanical stretch injury. Neurobiol Dis, 46(3), 646-654. PMID: 22426393

The α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) is an ionotropic transmembrane receptor for glutamate found throughout the central nervous system (CNS). AMPARs are composed of four types of subunits: GluR1/GluA1, GluR2/GluA2, GluR3/GluA3, and GluA4 (GluRA-D2), which combine to form heterotetramers.

Phosphorylation of AMPARs is often implicated in synaptic plasticity and the pathological signalling seen in several disease states. Phosphorylation of GluR1 at serine 845 has been implicated in specific neurological diseases including paediatric seizures. Phosphorylation of the S845 of the GluR1 subunit is controlled by cGKII, a kinase activated by an increase in nitric oxide (NO) levels within the cytosol, and PKA, activated by the calcium dependent production of cAMP. GluR1 S845 is dephosphorylated by the protein phosphatases PP1, PP2A, and CaN (also known as PP2B).

Anti-Phospho-GluR1 (pS845) antibody

Cat No. Pack Size Price Qty

crb2005048d

20ug

$165.00

crb2005048e

50ug

$330.00

crb2115048e

Alexa Fluor® 488

$405.00