Alexa Fluor® 488 Anti-Phospho-GluR1 (pS845) antibody

$405.00

Description

Recognises the GluR1 subunit of AMPAR with a phosphorylated serine at position 845. Antibody is conjugated to Alexa Fluor® 488 fluorescent dye

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Catalogue number crb2115048e
Antibody Anti-Phospho-GluR1 (pS845) antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200334e
Protein ID UniProtKB - P35438
Aliases GluA1
Cross-Reactivity Human, rat, mouse, (predicted) goat
Host Species Anti-Rabbit
Antibody Type Polyclonal
Label Labelled with Alexa Fluor® 488 under optimal conditions. Ex: 495nm Em:519nm
Concentration 1mg/ml
Validation ELISA
Target phosphorylated GluR1 (pS845)
Disease Area Neurodegenerative
Family AMPA receptor
Specificity Protein
Storage Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C
References

Khatib, T., Whiting, A., Chisholm, D., Redfern, C., Müller, B. and McCaffery, P. (2019). A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor. Mol Neurobiol. PMID: 30972628

 

Spaethling, J., Le, L. and Meaney, D. (2012). NMDA receptor mediated phosphorylation of GluR1 subunits contributes to the appearance of calcium-permeable AMPA receptors after mechanical stretch injury. Neurobiol Dis, 46(3), 646-654. PMID: 22426393

The α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) is an ionotropic transmembrane receptor for glutamate found throughout the central nervous system (CNS). AMPARs are composed of four types of subunits: GluR1/GluA1, GluR2/GluA2, GluR3/GluA3, and GluA4 (GluRA-D2), which combine to form heterotetramers.

Phosphorylation of AMPARs is often implicated in synaptic plasticity and the pathological signalling seen in several disease states. Phosphorylation of GluR1 at serine 845 has been implicated in specific neurological diseases including paediatric seizures. Phosphorylation of the S845 of the GluR1 subunit is controlled by cGKII, a kinase activated by an increase in nitric oxide (NO) levels within the cytosol, and PKA, activated by the calcium dependent production of cAMP. GluR1 S845 is dephosphorylated by the protein phosphatases PP1, PP2A, and CaN (also known as PP2B).

This antibody is conjugated to Alexa Fluor® 488. Alexa Fluor® 488 is a popular bright green fluorescent dye with high pH-stability.

Alexa Fluor® 488 Anti-Phospho-GluR1 (pS845) antibody