Anti-FGFR2b antibody
Description
Application Data
Description
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Recognises fibroblast growth factor receptor 2b epithelial splice variant, a receptor tyrosine kinase involved in cell growth and survival
Application Data
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FGFR2b-His and FGFR2c-His transfected HEK293 cells.
Lane 1: Conditioned media from non-transfected HEK293 cells
Lane 2: Conditioned media from HEK293 cells stably expressing soluble FGFR2b-His
Lane 3: Conditioned media from HEK293 cells stably expressing soluble FGFR2c-His
* Non-specific bovine albumin band from foetal calf serum media supplement
Western blot image provided courtesy of Dr. Jennet Gummadova
The fibroblast growth factor receptor (FGFR) family consists of four transmembrane receptor tyrosine kinases (FGFR1‐4), which regulate important physiological processes, such as cell proliferation, differentiation, migration and survival throughout the body. FGFR2b is known as the epithelial FGFR2 isoform. FGFR2b/KGFR is exclusively expressed in epithelial cells and can be activated by stimulation with its specific ligands FGF7/KGF or FGF10. Ligand binding results in a signalling cascade which includes phosphoinositide 3‐kinase (PI3K) and mitogen‐activated protein kinase (MAPK) signalling pathways and/or activation of phospholipase C-γ (Plc-γ). FGFR2b activation culminates in the survival, growth, and differentiation of epithelial cells, triggers phagocytosis and melanosome uptake in human keratinocytes and is critical for proper craniofacial development.
The fibroblast growth factor receptor (FGFR) family consists of four transmembrane receptor tyrosine kinases (FGFR1‐4), which regulate important physiological processes, such as cell proliferation, differentiation, migration and survival throughout the body. FGFR2b is known as the epithelial FGFR2 isoform. FGFR2b/KGFR is exclusively expressed in epithelial cells and can be activated by stimulation with its specific ligands FGF7/KGF or FGF10. Ligand binding results in a signalling cascade which includes phosphoinositide 3‐kinase (PI3K) and mitogen‐activated protein kinase (MAPK) signalling pathways and/or activation of phospholipase C-γ (Plc-γ). FGFR2b activation culminates in the survival, growth, and differentiation of epithelial cells, triggers phagocytosis and melanosome uptake in human keratinocytes and is critical for proper craniofacial development.
| Catalogue number | crb2005089 |
|---|---|
| Antibody | Anti-FGFR2b antibody |
| Antigen Peptide | KLH conjugated synthetic peptide crb1200370e |
| Protein ID | UniProtKB - |
| Aliases | Fibroblast growth factor receptor 2b, FGFR2b, Keratinocyte growth factor receptor, CD332 |
| Cross-Reactivity | Human |
| Host Species | Anti-Rabbit |
| Antibody Type | Polyclonal |
| Concentration | 1mg/ml |
| Validation | 1:100 (WB), 1:1000 (ELISA) |
| Target | FGFR2b |
| Disease Area | Signal transduction |
| Family | Tyrosine protein kinase family |
| Citations | MacKenzie, B., Henneke, I., Hezel, S., Al Alam, D., El Agha, E., Chao, C., Quantius, J., Wilhelm, J., Jones, M., Goth, K., Li, X., Seeger, W., Königshoff, M., Herold, S., Rizvanov, A., Günther, A. and Bellusci, S. (2015). Attenuating endogenous Fgfr2b ligands during bleomycin-induced lung fibrosis does not compromise murine lung repair. Am J Physiol Lung Cell Mol Physiol, 308(10), 1014-L1024. PMID: 25820524
Nanni, M., Ranieri, D., Raffa, S., Torrisi, M. and Belleudi, F. (2017). Interplay between FGFR2b-induced autophagy and phagocytosis: role of PLCγ-mediated signalling. J Cell Mol Med, 22(1), 668-683. PMID: 28994193 |
| References | MacKenzie, B., Henneke, I., Hezel, S., Al Alam, D., El Agha, E., Chao, C., Quantius, J., Wilhelm, J., Jones, M., Goth, K., Li, X., Seeger, W., Königshoff, M., Herold, S., Rizvanov, A., Günther, A. and Bellusci, S. (2015). Attenuating endogenous Fgfr2b ligands during bleomycin-induced lung fibrosis does not compromise murine lung repair. Am J Physiol Lung Cell Mol Physiol, 308(10), 1014-L1024. PMID: 25820524
Nanni, M., Ranieri, D., Raffa, S., Torrisi, M. and Belleudi, F. (2017). Interplay between FGFR2b-induced autophagy and phagocytosis: role of PLCγ-mediated signalling. J Cell Mol Med, 22(1), 668-683. PMID: 28994193 |
The fibroblast growth factor receptor (FGFR) family consists of four transmembrane receptor tyrosine kinases (FGFR1‐4), which regulate important physiological processes, such as cell proliferation, differentiation, migration and survival throughout the body. FGFR2b is known as the epithelial FGFR2 isoform.
FGFR2b/KGFR is exclusively expressed in epithelial cells and can be activated by stimulation with its specific ligands FGF7/KGF or FGF10. Ligand binding results in a signalling cascade which includes phosphoinositide 3‐kinase (PI3K) and mitogen‐activated protein kinase (MAPK) signalling pathways and/or activation of phospholipase C-γ (Plc-γ). FGFR2b activation culminates in the survival, growth, and differentiation of epithelial cells, triggers phagocytosis and melanosome uptake in human keratinocytes and is critical for proper craniofacial development.