Anti-ɣH2AX (pS139) antibody

Description

Recognises phosphorylated histone H2AX in Western blots and immunohistochemistry. Localises to sites of DNA damage to initiate DNA repair mechanisms

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Application Data

Catalogue number crb2005119
Antibody Anti-ɣH2AX (pS139) antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200386e
Protein ID UniProtKB - P16104
Aliases Phosphorylated Histone H2AX (pS139)
Cross-Reactivity Human, mouse
Host Species Anti-Rabbit
Antibody Type Polyclonal
Concentration 1mg/ml
Validation 1:1000 WB, 1:800 IF, 1:1000 ELISA
Target gamma H2AX (pS139)
Disease Area Histones, DNA damage
Family Histones
Post-translational Modification Phosphorylation of Serine 139
Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.
References

Feng et al., (2017). H2AX facilitates classical non-homologous end joining at the expense of limited nucleotide loss at repair junctions. Nucleic Acids Res, 45(18), 10614-10633. PMID: 28977657

 

Kuo and Yang (2008). Gamma-H2AX - a novel biomarker for DNA double-strand breaks. In Vivo, 22(3), 305-9. PMID: 18610740

Antibody which recognises the phosphorylated form of the H2A histone variant H2AX. H2AX is rapidly phosphorylated on serine 139 in response to DNA double strand breaks (DSBs). DSBs occur as part of the natural process of meiosis, and in response to external stimuli and mutagens. In either form, DSBs pose a huge threat to genome integrity and must be repaired quickly and accurately.

In mammalian cells, repair of DSBs is carried out primarily by either homologous recombination (HR) or non-homologous end joining (NHEJ). Upon DNA damage, phosphorylated H2AX, termed ‘γH2AX’, quickly accumulates and initiates a DNA damage signalling cascade at the DSB site. γH2AX also alters the chromatin at the DSB site to form an area accessible to the protein interactions and modifications required for DSB repair.

Anti-ɣH2AX can be seen to stain DSBs shortly after formation to form bright foci associated with the chromatin at the DSB site.

Anti-ɣH2AX (pS139) antibody

Cat No. Pack Size Price Qty

crb2005119d

20µg

$165.00

crb2005119f

100µg

$330.00