Anti-GLAST antibody

Description

Recognises the Glutamate-Aspartate Transporter (GLAST) in Western blots and immunohistochemistry with low background signal

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Application Data

Catalogue number crb2005009
Antibody Anti-GLAST Antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200276e
Protein ID UniProtKB - P24942
Aliases Anti-EAAT1 Antibody, Anti-Excitatory Amino Acid Transporter 1 Antibody, Anti-Glutamate Transporter Antibody, Anti-SLC1A3 Antibody
Cross-Reactivity Rat, Mouse
Host Species Rabbit
Antibody Type Polyclonal
Label Unconjugated
Concentration 1mg/ml
Validation WB/ELISA
Target Glutamate Transporter GLAST
Research Area Neurodegenerative diseases
Family sodium:dicarboxylate (SDF) symporter family.
Specificity Protein
Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.
References

Martin, C., Houitte, D., Guillermier, M., Petit, F., Bonvento, G. and Gurden, H. (2012). Alteration of sensory-evoked metabolic and oscillatory activities in the olfactory bulb of GLAST-deficient mice. Front Neural Circuits, 6. PMID: 22291618

 

Perkins, E., Clarkson, Y., Suminaite, D., Lyndon, A., Tanaka, K., Rothstein, J., Skehel, P., Wyllie, D. and Jackson, M. (2018). Loss of cerebellar glutamate transporters EAAT4 and GLAST differentially affects the spontaneous firing pattern and survival of Purkinje cells. Hum Mol Genet, 27(15), 2614-2627. PMID: 29741614

 

Siddiqi, F., Chen, F., Aron, A., Fiondella, C., Patel, K. and LoTurco, J. (2012). Fate Mapping by PiggyBac Transposase Reveals That Neocortical GLAST+ Progenitors Generate More Astrocytes Than Nestin+ Progenitors in Rat Neocortex. Cereb Cortex, 24(2), 508-520. PMID: 23118195

Santa_Cruz_alternative sc-7757, sc-7758
GLAST/ EAAT-1 (GLutamate–ASpartate Transporter/ Excitatory Amino Acid transporter 1) (rodent/human nomenclature) is a sodium-dependent plasma membrane glutamate transporter expressed exclusively by astrocytes in the cerebellum and present at high densities near excitatory synapses. Glutamate transporters regulate glutamate receptors and limit glutamate accumulation to prevent neurotoxicity whilst ensuring accurate synaptic communication. GLAST is the major transporter expressed during development.

Loss of GLAST/EAAT-1 has been linked to the pathogenesis of several disorders affecting the motor system including several subtypes of spinocerebellar ataxia (SCA); SCA1, SCA5, SCA7, episodic ataxia type 6, spinal muscular atrophy and fragile X associated tremor/ataxia syndrome. Furthermore, disrupted GLAST/EAAT-1 has been associated with schizophrenia and cerebellar dysfunction and also is linked to the pathophysiology of Alzheimer’s disease, autism and other cognitive and neuropsychiatric disorders.

Anti-GLAST antibody

Cat No. Pack Size Price Qty

crb2005009d

20ug

$150.00

crb2005009f

100ug

$300.00