Immunohistochemistry analysis of paraformaldehyde-fixed tissue sections from normal mouse kidney and Teratoma in mouse kidney. This was washed with PBST, 0.3% Triton X-100. The tissue was then blocked in 0.2%BSA+5%GNS+0.3%TritonX-100 in PBS for 1 hour at room temperature followed by incubation with primary antibodies overnight at 4 degrees (anti-Nanog 1:500).
Detection: DAB staining. Nuclei counterstained with Haemtoxylin
Image provided courtesy of Dr. Cheng Chen, Department of Developmental and Stem Cell Biology, Pasteur Institute .