crb2005295 Anti-OOEP antibody (L26) 

Glycine eluate:  1.33 µg/ml 

Molecular weight:  ~18.46 kDa

 Lane 1: Precision plus protein standard (BioRad) 

Lane 2:  mouse Oocyte

Western blot analysis of Anti-OOEP antibody using mouse oocytes  isolated from 8 week old FVB/N mice. Oocytes were isolated and washed through PBS. They were then snap frozen two times and lysed in 4X NuPAGE™ LDS Sample Buffer at 95ºC for 10 minutes. Samples were resolved on a 10-well NuPAGE 4 to 12% bis-tris protein gel of 1.0 mm thickness with NuPAGE MOPS SDS Running Buffer.1:1000 dilution of the primary antibody, in 5% BSA incubated at 4ºC overnight. And anti-rabbit HRP secondary antibody at 1:10000 (stock: 0.8 mg/mL), 1 hour RT. Developed with Pierce ECL Western Blotting Substrate. Images provided courtesy of Ida Jentoft, Max Planck Institute for Biophysical Chemistry.