Anti-OOEP antibody

Description

FLOPED/OOEP is an abundant protein in the oocyte cytoplasm, the absence of this protein leads to early embryonic arrest in both mice and humans

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Application Data

Catalogue number crb2005295
Antibody Anti-OOEP antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200531e
Protein ID UniProtKB - Q9CWE6
Aliases Oocyte-expressed protein homolog, OOEP , Factor located in oocytes permitting embryonic development, FLOPED, Oocyte- and embryo-specific protein 19, mOEP19
Cross-Reactivity Mouse
Host Species Rabbit
Antibody Type Polyclonal
Concentration 1 mg/ml Glycine eluate (L26)
Validation WB (1:1000), ELISA (I:1000), IF (1:1000)
Target mouse OOEP
Disease Area Oocyte biology, early developmental biology, Infertility, recurrent hydatidiform mole formation
Family KH domain RNA binding proteins
Super Family KHDC1 family
Specificity Recognises N-terminus
Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.
References

Li et al., (2008). A Subcortical Maternal Complex Essential for Pre-implantation Mouse Embryogenesis. Dev Cell. 15(3): 416. PMID: 18804437

 

Tashiro et al., (2010). Maternal‐effect gene Ces5/Ooep/Moep19/Floped is essential for oocyte cytoplasmic lattice formation and embryonic development at the maternal‐zygotic stage transition. Genes to cells. 15 (8). 813.

Factor located in oocytes permitting embryonic development (FLOPED)/OOEP, along with, MATER, TLE6 and Filia, forms the subcortical maternal complex (SCMC). The SCMC assembles during oocyte growth and is essential for zygotes to progress beyond the first embryonic cell divisions.

Floped expression is seen specifically in growing and mature oocytes and in early embryos, and is localised in the subcortex region.

FLOPED/OOEP is conserved across mammals, and mutations in the Floped gene have been linked to female infertility. Floped knockout mutant eggs lack the SCMC and these embryos do not progress beyond cleavage stage development. Therefore FLOPED/OOEP protein seems to have an important function around the zygotic stage and in the subsequent development of the preimplantation embryos. Floped/Ooep gene is considered a typical maternal‐effect gene.

Anti-OOEP antibody

Cat No. Pack Size Price Qty

crb2005295d

20ug

$165.00

crb2005295f

100ug

$330.00