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Anti-OOEP antibody

Anti-OOEP antibody

Description

FLOPED/OOEP is an abundant protein in the oocyte cytoplasm, the absence of this protein leads to early embryonic arrest in both mice and humans

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Application Data

Anti-OOEP antibody validated using mouse oocytes isolated from 8 week old FVB/N mice. Oocytes were fixed in 2% PFA (+ 100 mM Hepes, 50 mM EGTA (pH7), 10 mM MgSO₄, and 0.2% Triton-X-100) for 30 min at 37ºC. They were then extracted overnight at 4ºC in 0.2% Triton-X-100 in PBS prior to blocking and antibody incubations. Anti-OOEP antibody was used at a 1:1000 dilution (stock 1.33 mg/mL) in 5% BSA with 0.1% Triton-X-100 and samples were incubated at 4ºC overnight . Secondary antibody was goat-anti rabbit conjugated to AlexaFluor488 1:400 (stock 2mg/mL), incubated 1.5 hours at RT.

Images provided courtesy of Ida Jentoft, Max Planck Institute for Biophysical Chemistry.

1. Validation

Catalogue number	crb2005295
Antibody	Anti-OOEP antibody
Antigen Peptide	KLH conjugated synthetic peptide crb1200531e
Protein ID	UniProtKB - Q9CWE6
Aliases	Oocyte-expressed protein homolog, OOEP , Factor located in oocytes permitting embryonic development, FLOPED, Oocyte- and embryo-specific protein 19, mOEP19
Cross-Reactivity	Mouse
Host Species	Rabbit
Antibody Type	Polyclonal
Concentration	1 mg/ml Glycine eluate (L26)
Validation	WB (1:1000), ELISA (I:1000), IF (1:1000)
Target	mouse OOEP
Disease Area	Oocyte biology, early developmental biology, Infertility, recurrent hydatidiform mole formation
Family	KH domain RNA binding proteins
Super Family	KHDC1 family
Specificity	Recognises N-terminus
Storage	This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.
References	Li et al., (2008). A Subcortical Maternal Complex Essential for Pre-implantation Mouse Embryogenesis. Dev Cell. 15(3): 416. PMID: 18804437 Tashiro et al., (2010). Maternal‐effect gene Ces5/Ooep/Moep19/Floped is essential for oocyte cytoplasmic lattice formation and embryonic development at the maternal‐zygotic stage transition. Genes to cells. 15 (8). 813.

Factor located in oocytes permitting embryonic development (FLOPED)/OOEP, along with, MATER, TLE6 and Filia, forms the subcortical maternal complex (SCMC). The SCMC assembles during oocyte growth and is essential for zygotes to progress beyond the first embryonic cell divisions.

Floped expression is seen specifically in growing and mature oocytes and in early embryos, and is localised in the subcortex region.

FLOPED/OOEP is conserved across mammals, and mutations in the Floped gene have been linked to female infertility. Floped knockout mutant eggs lack the SCMC and these embryos do not progress beyond cleavage stage development. Therefore FLOPED/OOEP protein seems to have an important function around the zygotic stage and in the subsequent development of the preimplantation embryos. Floped/Ooep gene is considered a typical maternal‐effect gene.

Anti-OOEP antibody

Cat No.	Pack Size	Price	Qty
crb2005295d	20ug	$165.00
crb2005295f	100ug	$330.00

Anti-OOEP antibody

Description

Application Data

1. Validation

Anti-OOEP antibody

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