Anti-OTULIN antibody

Description

Recognises the methionine 1 (M1)-specific deubiquitinase, OTULIN, a regulator of immune homeostasis

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Application Data

Catalogue number crb2005228
Antibody Anti-OTULIN antibody
Antigen Peptide Protein
Protein ID UniProtKB-Q96BN8
Aliases Ubiquitin thioesterase otulin, FAM105B
Cross-Reactivity Human
Host Species Rabbit
Antibody Type Polyclonal
Concentration serum
Validation 1:1000 (WB), 1:1000 (ELISA)
Target OTULIN
Specificity Protein
Storage The product should be stored at -20°C for short term storage and long term storage. Avoid repeated freeze/ thaw cycles.
References

Damgaard, R., Walker, J., Marco-Casanova, P., Morgan, N., Titheradge, H., Elliott, P., McHale, D., Maher, E., McKenzie, A. and Komander, D. (2016). The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Cell, 166(5), 1215-1230.e20. PMID: 27523608

 

Elliott, P. and Komander, D. (2015). Regulation of Met1-linked polyubiquitin signalling by the deubiquitinase OTULIN. FEBS J, 283(1), 39-53. PMID: 26503766

 

Weber, A., Elliott, P., Pinto-Fernandez, A., Bonham, S., Kessler, B., Komander, D., El Oualid, F. and Krappmann, D. (2017). A Linear Diubiquitin-Based Probe for Efficient and Selective Detection of the Deubiquitinating Enzyme OTULIN. Cell Chem Biol, 24(10), 1299-1313.e7. PMID: 28919039

The methionine 1 (M1)-specific deubiquitinase (DUB), OTULIN (linear ubiquitination-specific deubiquitinase ovarian tumour domain deubiquitinase with linear linkage specificity), acts as a negative regulator of nuclear factor κB signalling and immune homeostasis. OTULIN cleaves M1-linked polyUb with high specificity and efficiency and thus tightly regulates the linear ubiquitin chain assembly complex (LUBAC) which is responsible for catalysing the conjugation of linear methionine 1 (M1)-linked polyubiquitin (polyUb).

OTULIN is also a negative regulator of tumour necrosis factor- α (TNF-α) signalling, it prevent auto-ubiquitination of LUBAC components at steady state, and the accumulation of M1-linked chains. OTULIN is critical for preventing spontaneous inflammation and maintaining immune homeostasis.

Anti-OTULIN antibody

Cat No. Pack Size Price Qty

crb2005228d

20µL

$165.00

crb2005228f

100µL

$330.00