Anti-Phospho-CDK2 (pY15) antibody

Description

Recognises CDK2 phosphorylated on tyrosine 15

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Catalogue number crb2005014
Antibody Anti-Phospho-CDK2 (pY15) antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200296e
Protein ID UnitProtKB - P24941
Aliases Cyclin-dependent kinase 2, CDK2, CDK-2, Cell division protein kinase 2, p33 protein kinase
Cross-Reactivity Human, rat, mouse
Host Species Rabbit
Antibody Type Polyclonal
Concentration 1mg/ml
Validation WB/ELISA /IHC
Target phosphorylated CDK2 (pY15)
Research Area Cancer
Family Ser/Thr protein kinases (cyclin-dependent)
Specificity Protein
Storage Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C.
References

Hughes, B., Sidorova, J., Swanger, J., Monnat, R. and Clurman, B. (2013). Essential role for Cdk2 inhibitory phosphorylation during replication stress revealed by a human Cdk2 knockin mutation. PNAS, 110(22), 8954-8959.

 

PMID: 23671119 Ji, X., Humenik, J., Yang, D. and Liebhaber, S. (2017). PolyC-binding proteins enhance expression of the CDK2 cell cycle regulatory protein via alternative splicing. Nucleic Acids Res, 46(4), 2030-2044. PMID: 29253178

Cyclin-dependent kinases (CDKs) are regulated by positive and negative phosphorylation. T14/Y15 phosphorylation by the WEE1 and MYT1 kinases inhibits CDKs by preventing ATP binding, and these conserved phosphorylations are highly regulated during the cell cycle and by signalling pathways. WEE1 and MYT1 are opposed by cell division cycle 25 (CDC25) phosphatases, which, in mammals, comprise three related enzymes that dephosphorylate T14/Y15 to promote CDK activity.

Cdk2 inhibition is involved in DNA replication and S-phase DNA damage checkpoints. CDK2 T14/Y15 phosphorylation is not required for DNA damage-induced cell cycle arrest, but instead has roles in S-phase entry and centrosome duplication.

Anti-Phospho-CDK2 (pY15) antibody

Cat No. Pack Size Price Qty

crb2005014d

20ug

$150.00

crb2005014e

100ug

$300.00