Anti-phospho-FoxO1 (pS218) antibody

Description

Recognises FoxO1 transcriptional regulator phosphorylated at serine 218 in Western blots and immunohistochemistry with a strong signal and low background noise

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Application Data

Catalogue number crb2005226
Antibody Anti-phospho-FoxO1 (pS218) antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200468e
Protein ID UniProtKB - Q12778
Cross-Reactivity Human, rat, mouse. May also recognise the equivalent serine in FoxO3/4/6
Host Species Anti-Rabbit
Antibody Type Polyclonal
Concentration 0.5 mg/ml
Validation WB (1:500), ELISA (1:1000), IHC (1:100)
Target phosphorylated FoxO1 (pS218)
Citations Riddell, M., Nakayama, A., Hikita, T., Mirzapourshafiyi, F., Kawamura, T., Pasha, A., Li, M., Masuzawa, M., Looso, M., Steinbacher, T., Ebnet, K., Potente, M., Hirose, T., Ohno, S., Fleming, I., Gattenlöhner, S., Aung, P., Phung, T., Yamasaki, O., Yanagi, T., Umemura, H. and Nakayama, M. (2018). aPKC controls endothelial growth by modulating c-Myc via FoxO1 DNA-binding ability. Nature Comm, 9(1). PMID: 30559384
Research Area Cancer, Cell signalling, Metabolism
Specificity Protein
Storage The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles.
References

Wilhelm, K., Happel, K., Eelen, G., Schoors, S., Oellerich, M., Lim, R., Zimmermann, B., Aspalter, I., Franco, C., Boettger, T., Braun, T., Fruttiger, M., Rajewsky, K., Keller, C., Brüning, J., Gerhardt, H., Carmeliet, P. and Potente, M. (2016). FOXO1 couples metabolic activity and growth state in the vascular endothelium. Nature, 529(7585), 216-220. PMID: 26735015

Forkhead box O1 (FoxO1) is a transcriptional regulator of cell growth and proliferation controlled by a receptor tyrosine kinase signalling pathway (the vascular endothelial growth factor A (VEGF-A)/phosphatidylinositol-3-OH kinase (PI3K)/protein kinase B (PKB/Akt) pathway). FoxO1 is phosphorylated in the DNA binding domain at serine 218 by aPKCλ. FoxO1 Ser218 phosphorylation and aPKC expression correlate with poor patient prognosis in various cancers.

VEGF/PI3K/Akt signalling results in FoxO1 cytoplasmic localisation and its inactivation. Cytoplasmically localised FoxO1 is associated with cellular myelocytomatosis (c-Myc) expression and endothelial cell (EC) proliferation. Abnormal atypical protein kinase C (aPKC)/FoxO1/c-Myc signalling contributes to excessive EC proliferation in angiosarcoma and when constitutively active, FoxO1 leads to lethality due to heart defects.

Anti-phospho-FoxO1 (pS218) antibody

Cat No. Pack Size Price Qty

crb2005226d

20ug

$150.00

crb2005226f

100ug

$300.00