Anti-T2A antibody

Description

Recognises the 2A peptide from foot-and-mouth disease virus and can detect 2A tagged recombinant proteins

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Catalogue number crb2005269
Antibody Anti-T2A antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200519e
Aliases T2A, Thosea asigna virus 2A
Cross-Reactivity T2A tag
Host Species Rabbit
Antibody Type Polyclonal
Concentration 0.5mg/ml Glycine, 0.2mg/ml TEA (Rb L02T), 0.5mg/ml Glycine (Rb L02G)
Validation 1:1000 (ELISA)
Target T2A
Specificity T2A
Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.
Citations Ferreira, A.C.F., Szeto, A.C.H., Heycock, M.W.D. et al. RORα is a critical checkpoint for T cell and ILC2 commitment in the embryonic thymus. Nat Immunol (2021). https://doi.org/10.1038/s41590-020-00833-w
References

Hofacre et al., (2018). Efficient Therapeutic Protein Expression Using Retroviral Replicating Vector with 2A Peptide in Cancer Models. Hum Gen Ther, 29(4), 437-451. PMID: 29216761

 

Sun et al., (2017). Processing and targeting of proteins derived from polyprotein with 2A and LP4/2A as peptide linkers in a maize expression system. PLOS ONE, 12(3), e0174804. PMID: 28358924

2A peptide-linked polycistronic vectors can be used to express multiple proteins from a single open reading frame (ORF). The small 2A peptide sequences, when cloned between genes, allows for efficient, stoichiometric production of discrete protein products within a single vector through a novel "cleavage" event within the 2A peptide sequence.

2A was discovered in the foot-and-mouth disease virus (FMDV, a picornavirus). The 2A peptide acts through ribosomal skipping to allow for the encoding of polyproteins which can dissociate into individual proteins upon translation. Anti-T2A antibody recognises 2A tagged recombinant proteins and is an excellent tool for researchers using 2A peptide based expression systems.

Anti-T2A antibody

Cat No. Pack Size Price Qty

crb2005269d

20ug

$165.00

crb2005269d/L02T

L02T 20ug

$165.00

crb2005269f/L02T

L02T 100ug

$330.00

crb2005269d/L02G

L02G 20ug

$165.00

crb2005269f/L02G

L02G 100ug

$330.00