Anti-T2A antibody

Description

Recognises the 2A peptide from foot-and-mouth disease virus and can detect 2A tagged recombinant proteins

See full description

Catalogue number crb2005269
Antibody Anti-T2A antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200516e
Aliases T2A, Thosea asigna virus 2A
Cross-Reactivity T2A tag
Host Species Rabbit
Antibody Type Polyclonal
Concentration 0.5 mg/ml
Validation 1:1000 (ELISA)
Target T2A
Specificity T2A
Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.
References

Hofacre, A., Yagiz, K., Mendoza, D., Lopez Espinoza, F., Munday, A., Burrascano, C., Singer, O., Gruber, H., Jolly, D. and Lin, A. (2018). Efficient Therapeutic Protein Expression Using Retroviral Replicating Vector with 2A Peptide in Cancer Models. Hum Gen Ther, 29(4), 437-451. PMID: 29216761

 

Sun, H., Zhou, N., Wang, H., Huang, D. and Lang, Z. (2017). Processing and targeting of proteins derived from polyprotein with 2A and LP4/2A as peptide linkers in a maize expression system. PLOS ONE, 12(3), e0174804. PMID: 28358924

2A peptide-linked polycistronic vectors can be used to express multiple proteins from a single open reading frame (ORF). The small 2A peptide sequences, when cloned between genes, allows for efficient, stoichiometric production of discrete protein products within a single vector through a novel "cleavage" event within the 2A peptide sequence.

2A was discovered in the foot-and-mouth disease virus (FMDV, a picornavirus). The 2A peptide acts through ribosomal skipping to allow for the encoding of polyproteins which can dissociate into individual proteins upon translation. Anti-T2A antibody recognises 2A tagged recombinant proteins and is an excellent tool for researchers using 2A peptide based expression systems.

Anti-T2A antibody

Cat No. Pack Size Price Qty

crb2005269d

20ug

$150.00

crb2005269f

100ug

$300.00

Out of stock