Anti-2SC antibody

Description

Detects succinated cysteine residues on succinated proteins with high affinity

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Application Data

Catalogue number crb2005017
Antibody Anti-2SC antibody
Antigen Peptide KLH conjugated S-(2-succinyl)-cysteine
Aliases S-(2-succinyl)-cysteine
Host Species Anti-Rabbit
Antibody Type Polyclonal
Concentration 0.5mg/ml TEA eluate (Rb 6773), 0.4mg/ml TEA (Rb 6772), 0.4mg/ml Glycine (Rb 6774)
Validation Rb 6773: WB (1:200), ELISA (I:1000), IHC (1:2000), Rb 6772: IHC (1:400), Rb 6774: IHC (1:2000)
Target 2SC
Disease Area Cancer Biology, Diabetes, Obesity, Renal cancer
Specificity Succinated cysteine residues
Post-translational Modification Succination
Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.
Citations

Burgener et al., (2019). SDHA gain-of-function engages inflammatory mitochondrial retrograde signaling via KEAP1-Nrf2. Nat Immunol. 20(10):1311-1321. PMID: 31527833

Casey et al., (2020). Fumarate Metabolic Signature for the Detection of Reed Syndrome in Humans.Clin Cancer Res.  26(2):391-396. PMID: 31636096. doi: 10.1158/1078-0432.

Gupta et al., (2020). Secondary Renal Neoplasia Following Chemotherapy or Radiation in Pediatric Patients. Hum Pathol.  PMID: 32681944. doi: 10.1016/j.humpath.2020.07.014.

Äyräväinen et al., (2020). Systematic molecular and clinical analysis of uterine leiomyomas from fertile-aged women undergoing myomectomy. Human Reproduction. deaa187, https://doi.org/10.1093/humrep/deaa187

 

References

Alderson, N., Wang, Y., Blatnik, M., Frizzell, N., Walla, M., Lyons, T., Alt, N., Carson, J., Nagai, R., Thorpe, S. and Baynes, J. (2006). S-(2-Succinyl)cysteine: A novel chemical modification of tissue proteins by a Krebs cycle intermediate. Arch Biochem Biophys, 450(1), 1-8. PMID: 16624247

 

Nagai, R., Brock, J., Blatnik, M., Baatz, J., Bethard, J., Walla, M., Thorpe, S., Baynes, J. and Frizzell, N. (2007). Succination of Protein Thiols during Adipocyte Maturation: a biomarker of mitochondrial stress. J Biol Chem, 282(47), 34219-34228. PMID: 17726021

 

Merkley, E., Metz, T., Smith, R., Baynes, J. and Frizzell, N. (2013). The succinated proteome. Mass Spectrom Rev, 33(2), 98-109. PMID: 24115015

 

Succination is a stable post-translational modification of cysteine residues, which modifies protein function or turnover in response to a changing intracellular redox environment. Succination occurs when the Krebs cycle intermediate, fumarate, reacts with cysteine yielding S-(2-succino)cysteine (2SC).

A wide range of proteins are subject to succination, including enzymes, adipokines, cytoskeletal proteins and ER chaperones and succination has been shown to have roles in regulatory biology.

An increase in succination of adipocyte proteins is seen in diabetes mellitus and results from nutrient excess derived mitochondrial stress. 2SC therefore serves as a biomarker of mitochondrial stress or dysfunction in chronic diseases, such as obesity, diabetes, and cancer.

Anti-2SC antibody

Cat No. Pack Size Price Qty

crb2005017d/6772

6772 20ug

$330.00

crb2005017e/6772

6772 50ug

$480.00

crb2005017d/6773

6773 20ug

$420.00

crb2005017e/6773

6773 50ug

$600.00

crb2005017d/6774

6774 20ug

$330.00

crb2005017e/6774

6774 50ug

$480.00