Alexa Fluor® 488 Anti-GLAST antibody

$375.00

Description

Recognises the glutamate transporter, GLAST, in Western blots and immunohistochemistry with low background signal. Has Alexa Fluor® 488 fluorescent dye attached

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Catalogue number crb2115009e
Antibody crb2115009e Alexa Fluor® 488 Anti-Glutamate Transporter (GLAST) antibodyAnti-GLAST Antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200276e
Protein ID UniProtKB - P24942
Aliases SLC1A3, Solute carrier family 1 (glial high-affinity glutamate transporter), member 3, GLAST GLutamate ASpartate Transporter, EAAT1 Excitatory Amino Acid Transporter 1
Cross-Reactivity Rat, Mouse
Host Species Anti-Rabbit
Antibody Type Polyclonal
Label Labelled with Alexa Fluor® 488 under optimal conditions. Ex: 495nm Em:519nm
Concentration 0.56mg/ml
Validation ELISA
Target GLAST
Research Area Neurodegenerative diseases
Family sodium:dicarboxylate (SDF) symporter family.
Specificity Protein
Storage Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C
References

Martin, C., Houitte, D., Guillermier, M., Petit, F., Bonvento, G. and Gurden, H. (2012). Alteration of sensory-evoked metabolic and oscillatory activities in the olfactory bulb of GLAST-deficient mice. Front Neural Circuits, 6. PMID: 22291618

 

Perkins, E., Clarkson, Y., Suminaite, D., Lyndon, A., Tanaka, K., Rothstein, J., Skehel, P., Wyllie, D. and Jackson, M. (2018). Loss of cerebellar glutamate transporters EAAT4 and GLAST differentially affects the spontaneous firing pattern and survival of Purkinje cells. Hum Mol Genet, 27(15), 2614-2627. PMID: 29741614

 

Siddiqi, F., Chen, F., Aron, A., Fiondella, C., Patel, K. and LoTurco, J. (2012). Fate Mapping by PiggyBac Transposase Reveals That Neocortical GLAST+ Progenitors Generate More Astrocytes Than Nestin+ Progenitors in Rat Neocortex. Cereb Cortex, 24(2), 508-520. PMID: 23118195

GLAST/ EAAT-1 (glutamate–aspartate transporter/ excitatory amino acid transporter 1) (rodent/human nomenclature) is a sodium-dependent plasma membrane glutamate transporter expressed exclusively by astrocytes in the cerebellum and present at high densities near excitatory synapses. The cerebellum is the region of the brain essential for maintaining postural control and coordination of voluntary muscle movement.

Glutamate transporters regulate glutamate receptors and limit glutamate accumulation to prevent neurotoxicity whilst ensuring accurate synaptic communication. GLAST is the major transporter expressed during development.

Loss of GLAST/EAAT-1 has been linked to the pathogenesis of several disorders affecting the motor system including several subtypes of spinocerebellar ataxia (SCA); SCA1, SCA5, SCA7, episodic ataxia type 6, spinal muscular atrophy and fragile X associated tremor/ataxia syndrome. Furthermore, disrupted GLAST/EAAT-1 has been associated with schizophrenia and cerebellar dysfunction and also is linked to the pathophysiology of Alzheimer’s disease, autism and other cognitive and neuropsychiatric disorders.

This antibody has Alexa Fluor® 488 conjugated to it. Alexa Fluor® 488 is a popular bright green fluorescent dye with high PH-stability.

Alexa Fluor® 488 Anti-GLAST antibody