Alexa Fluor® 488 Anti-Cyclin D1 antibody

$375.00

Description

Recognises Cyclin D1, a key regulator of cell proliferation and tumourigenesis. Antibody is conjugated to Alexa Fluor® 488 fluorescent dye

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Catalogue number crb2115024e
Antibody Alexa Fluor® 488 Anti-Cyclin D1 antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200305e
Protein ID UnitProtKB - P24385
Aliases CCND1
Cross-Reactivity Human
Host Species Anti-Rabbit
Antibody Type Polyclonal
Label Labelled with Alexa Fluor® 488 under optimal conditions. Ex: 495nm Em:519nm
Concentration 0.53mg/ml
Validation ELISA
Target Cyclin D1
Research Area Cancer, cell cycle
Family Cyclin
Specificity Protein
Storage Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C
References

Pysz, M., Hao, F., Hizli, A., Lum, M., Swetzig, W., Black, A. and Black, J. (2014). Differential Regulation of Cyclin D1 Expression by Protein Kinase C α and ϵ Signaling in Intestinal Epithelial Cells. J Biol Chem, 289(32), 22268-22283. PMID: 24914206

 

Qie, S. and Diehl, J. (2016). Cyclin D1, cancer progression, and opportunities in cancer treatment. J Mol Med, 94(12), 1313-1326. PMID: 27695879

Cyclin D1 is a key regulator of cell proliferation and its expression and accumulation within cells is under tight control. Cyclin D1 is the regulatory subunit of cyclin-dependent kinases 4 and 6. These activated cyclin dependent kinases then phosphorylate the retinoblastoma protein (Rb) and drive G1 to S phase progression. Cyclin D1 promotes cell proliferation through interaction with transcription factors such as the oestrogen receptor and specificity protein 1 (Sp1). Cell proliferation is extremely sensitive to altered levels of cyclin D1, with even modest changes in its expression having noticeable effects on cell cycle progression. Cyclin D1 is a proto-oncogene and its overexpression is one of the most frequent alterations seen in multiple cancer types.

This antibody is conjugated to Alexa Fluor® 488. Alexa Fluor® 488 is a popular bright green fluorescent dye with high pH-stability.

Alexa Fluor® 488 Anti-Cyclin D1 antibody