Alexa Fluor® 488 Anti-ERK1/2 antibody

$375.00

Description

Recognises ERK1/2 a MAPK family protein which controls cell proliferation. Antibody is conjugated to Alexa Fluor® 488 fluorescent dye

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Catalogue number crb2115025e
Antibody Alexa Fluor® 488 Anti-ERK1/2 antibody (Rabbit Polyclonal IgG)
Antigen Peptide KLH conjugated synthetic peptide crb1200306e
Protein ID UniProtKB - P27361
Aliases Extracellular Signal-Related Kinase 1, ERK1, ERK-1, ERK2, ERK-2, Mitogen-Activated Protein Kinase 3, MAP Kinase 3, MAPK 3.
Cross-Reactivity Human, rat, mouse
Host Species Anti-Rabbit
Antibody Type Polyclonal
Label Labelled with Alexa Fluor® 488 under optimal conditions. Ex: 495nm Em:519nm
Concentration 0.67mg/ml
Validation ELISA
Target ERK1/2
Family Mitogen-activated protein (MAP) kinases family
Specificity ERK1 and ERK2
Storage Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C
References

Cook, S., Stuart, K., Gilley, R. and Sale, M. (2017). Control of cell death and mitochondrial fission by ERK1/2 MAP kinase signalling. FEBS J, 284(24), 4177-4195. PMID: 28548464

 

Zou, J., Lei, T., Guo, P., Yu, J., Xu, Q., Luo, Y., Ke, R. and Huang, D. (2018). Mechanisms shaping the role of ERK1/2 in cellular senescence (Review). Mol Med Rep, 19(2), 759–770. PMID: 30535440

Extracellular signal-regulated kinase 1/2 (ERK1/2) is a mitogen-activated protein kinase (MAPK) family protein and part of the Ras-Raf‐MEK‐ERK signalling pathway which plays a key role in controlling cell proliferation, differentiation and cell survival. ERK1/2 acts downstream of activated growth factor receptors, RAF protein kinases and mitogen-activated protein kinase kinases 1 and 2 (MEK1/2). MEK1 and MEK2 activate ERK1/2 by phosphorylation and once activated ERK1/2 enters the nucleus and phosphorylates transcription factors to induce changes in gene expression. In addition to this active ERK1/2 also translocates to other organelles including the endoplasmic reticulum, endosomes, golgi and mitochondria where it influences cell physiology. Overall ERK1/2 phosphorylates more than 200 different substrates including other protein kinases, transcription factors, RNA‐binding proteins, regulators of mRNA translation and regulators of cell death. ERK1/2 pathway is strongly implicated in cancer where its hyperactivation underpins the growth and maintenance of many tumour types.

This antibody is conjugated to Alexa Fluor® 488. Alexa Fluor® 488 is a popular bright green fluorescent dye with high pH-stability.

Alexa Fluor® 488 Anti-ERK1/2 antibody