Alexa Fluor® 488 Anti-HAND-1 antibody



Recognises HAND1, a twist family bHLH transcription factor essential for several developmental processes. Conjugated to AF488, a bright green fluorescent dye

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Catalogue number crb2115015e
Antibody Alexa Fluor® 488 Anti-HAND-1 antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200298f
Protein ID UniProtKB - O96004
Cross-Reactivity Human, Rat, Mouse
Host Species Anti-Sheep
Antibody Type Polyclonal
Concentration 0.69mg/ml
Validation ELISA
Target HAND-1
Disease Area Cardiovascular
Family Basic helix-loop-helix
Specificity Protein
Storage Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C.

Barnes, R. and Firulli, A. (2009). A twist of insight - the role of Twist-family bHLH factors in development. Int J Dev Biol, 53(7), 909-924. PMID: 19378251

Firulli, B., Fuchs, R., Vincentz, J., Clouthier, D. and Firulli, A. (2014). Hand1 phosphoregulation within the distal arch neural crest is essential for craniofacial morphogenesis. Development, 141(15), 3050-3061. PMID: 25053435

Heart and neural crest derivatives expressed 1 (HAND1) is a basic helix-loop-helix (bHLH) transcription factor belonging to the twist family. Twist-family transcription factors play essential roles in a number of developmental programs and function by dimerising with other bHLH members and binding to cis- regulatory elements, called E-boxes. HAND1 can form homodimers as well as interact with other tissue restricted bHLH proteins, such as HAND2. Dimer partner choice infers differential regulation on twist family proteins and is regulated by phosphorylation. Protein Kinase A and C (PKA and PKC) can phosphorylate HAND1, while β56δ-containing protein phosphatase 2A (PP2A) can dephosphorylate HAND1.

HAND1 is required for proper cardiac morphogenesis and is essential for extra-embryonic and trophoblast cell differentiation. Hand1 is expressed in several foetal tissues and in adult heart tissue where it may have a role in preventing hypertrophy.

This antibody has Alexa Fluor® 488 conjugated to it. Alexa Fluor® 488 is a popular bright green fluorescent dye with high PH-stability.

Alexa Fluor® 488 Anti-HAND-1 antibody