Anti-Grip71 antibody

Description

Recognises Grip71, a component of the γ-tubulin ring complex (γ-TuRC) from Drosophila in Western blots and immunohistochemistry

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Application Data

Catalogue number crb2005268
Antibody Anti-Grip71 antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200507e
Protein ID UniProtKB - Q9VJ57_DROME
Aliases DGrip71, Dgp71WD
Cross-Reactivity Drosophila
Host Species Rabbit
Antibody Type Polyclonal
Validation WB (1:1000), ELISA (1:1000), Immunoprecipitation
Target Grip71-PA (single isoform gene)
Citations Tovey, C., Tubman, C., Hamrud, E., Zhu, Z., Dyas, A., Butterfield, A., Fyfe, A., Johnson, E. and Conduit, P. (2018). γ-TuRC Heterogeneity Revealed by Analysis of Mozart1. Current Biology, 28(14), pp.2314-2323.e6
Research Area Cytoskeleton, Microtubles, gamma-tubulin ring complex
Family Grip71/NEDD1/GCPWD
Specificity Protein
Storage The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles.
References

Gunawardane, R., Martin, O. and Zheng, Y. (2003). Characterization of a New γTuRC Subunit with WD Repeats. Mol Biol Cell, 14(3), 1017-1026. PMID: 12631720

 

Romé, P. and Ohkura, H. (2018). A novel microtubule nucleation pathway for meiotic spindle assembly in oocytes. J Cell Biol, 217(10), 3431-3445. PMID: 30087124

Grip71 was first identified in Drosophila and named Drosophila gamma ring protein with WD repeats (Dgp71WD). It is a component of the γ-tubulin ring complex (γ-TuRC) and is a homologue of the mammalian NEDD1 (alternatively named GCP-WD). Despite the name, Grip71 lacks Grip domains and instead contains N-terminal WD40 repeats and a C-terminal domain with unknown structure required for Grip71’s association with the γ-TuRC. Grip71 recruits γ-TuRC to different microtubule organising centres (MTOCs).

Grip71 is recruited to the spindle equator and poles by Augmin. In mitotic cells, a loss of Grip71 or Augmin leads to a dramatic decrease in the spindle microtubule density.

Anti-Grip71 antibody

Cat No. Pack Size Price Qty

crb2005268d

20ug

$150.00

crb2005268f

100ug

$300.00