Anti-Histone H3.3 (G34V) antibody

Description

Recognises glycine to valine substitution mutation of histone variant H3.3 in Western blots and immunohistochemistry with strong signal and low background noise

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Catalogue number crb2005186
Antibody Anti-Histone H3 (G34V) antibody
Antigen Peptide KLH conjugated synthetic peptide crb1200377e
Protein ID UniProtKB - P84243
Aliases H3 Histone Family Member 3A, H3 Histone, H3.3A H3F3, Histone H3.3, H3F3A
Cross-Reactivity Human, Rabbit, Mouse
Host Species Rabbit
Antibody Type Polyclonal
Concentration 1mg/ml
Validation 1:1000 (WB), 1:1000 (ELISA), IF, IHC
Target Histone H3 (H3.3)
Disease Area Histones, Tumourigenesis
Family Histones
Specificity Protein
Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.
Citations Haque et al. (2017) Acta Neuropathol Commun.5(1):45. PMID: 28587626
References

Kallappagoudar, S., Yadav, R., Lowe, B. and Partridge, J. (2015). Histone H3 mutations—a special role for H3.3 in tumorigenesis? Chromosoma, 124(2), 177-189. PMID: 25773741

Voon, H., Udugama, M., Lin, W., Hii, L., Law, R., Steer, D., Das, P., Mann, J. and Wong, L. (2018). Inhibition of a K9/K36 demethylase by an H3.3 point mutation found in paediatric glioblastoma. Nature Commun, 9(1). PMID: 30087349

Wu, G., Broniscer, A., McEachron, T., Lu, C., Paugh, B., Becksfort, J., Qu, C., Ding, L., Huether, R., Parker, M., Zhang, J., Gajjar, A., Dyer, M., Mullighan, C., Gilbertson, R., Mardis, E., Wilson, R., Downing, J., Ellison, D., Zhang, J. and Baker, S. (2012). Somatic histone H3 alterations in pediatric diffuse intrinsic pontine gliomas and non-brainstem glioblastomas. Nature Genet, 44(3), 251-253. PMID: 22286216

Histone H3.3 is a replication-independent histone variant, which replaces canonical histone H3.1/2 outside of S phase. H3.3 is expressed throughout the cell cycle, as well as in quiescent cells and is referred to as the “replacement histone”. H3.3 is largely deposited in a DNA synthesis-independent fashion by a distinct set of chaperones proteins. H3.3 accumulates in post mitotic cells, such as cerebral cortical neurons and is incorporated at sites of UV damage where it protects against sensitivity to UV light.

H3.3 deposition occurs on DNA sequences that are transiently nucleosome-free, such as during transcription and DNA repair, therefore serving as a marker for regions of high transcriptional activity. H3.3 is also enriched in heterochromatic subtelomeric and pericentromeric regions. H3.3 plays important roles in many developmental contexts such as in stem cells, during fertilization and reproduction and during reprogramming of genomes following fertilization or somatic cell nuclear transfer.

Mutations in histone H3.3 are common events in certain cancers. H3.3 G34V (glycine to valine) substitution mutation is found in paediatric high-grade glioblastoma (HGG).

Anti-Histone H3.3 (G34V) antibody

Cat No. Pack Size Price Qty

crb2005186d

Anti-Histone H3 (G34V) antibody 20ug

$150.00

crb2005186f

Anti-Histone H3 (G34V) antibody 100ug

$300.00